Fig. 7: Integrin-based subcellular adhesion structures on RGD and LDVP surfaces.
From: Single-molecule characterization of subtype-specific β1 integrin mechanics
Fab (100 nM for HP1/7 and 20 nM for the others) and Sir-actin (10 nM) were added 10 min before live-cell imaging of cells seeded for 1 h. a Integrin αV (AF488-13C2 Fab, green in the merged images) and actin stress fibers (SiR-actin, red in the merged images) on RGD-54pN. Intensity profiles (bottom right panel) are along the dashed line in the third enlarged image. Arbitrary units (a.u.). b Integrin α4 (AF488-HP1/7 Fab) and actin stress fibers (SiR-actin) on LDVP-54pN. c Integrin α4 (Cy3-HP1/7 Fab) and αV (AF647-13C2 Fab). d Paxillin (AF488, green) immunostaining of fixed cells imaged with SiR-actin (red) and αV (Cy3-13C2 Fab) or α4 (Cy3-HP1/7 Fab). e Extended open β1 (Cy3-12G10 Fab) and closed β1 (AF647-mAb13 Fab) on RGD-54pN. f Extended open β1 (Cy3-12G10 Fab) and closed β1 (AF647-mAb13 Fab) on LDVP-54pN. Boxed regions are magnified in the bottom row or right column. Scale bars: 10 µm (white), 2 µm (yellow). Source data are provided as a Source Data file.
