Fig. 9: Integrin α5β1 mediated cell spreading.
From: Single-molecule characterization of subtype-specific β1 integrin mechanics
a Affinity of ACRGD for integrins on intact cells. Titration curves are shown in Supplementary Fig. 13. b–d Behavior of BJ-5ta on ACRGD-54pN (BHQ2-Cy3) surfaces after 1 h in presence of integrin blocking Fabs described in Fig. 6 legend. b Close-contact enclosed area from RICM images (n = 43, 38, 47, 96, 50 cells). c Aspect ratio of contact area (n = 56, 49, 38 cells, >500 μm2). d Ruptured TGT counts (n = 44, 40, 48, 96 cells). e–g Cell spreading on ACRGD-TGT of different Ttol in presence of integrin blocking Fabs. e Representative images of cells with RICM (top row) and same cells with DIC or Cy3 fluorescence to show TGT rupture (bottom row). Dim RICM rings (~20 μm diameter) with 12, 23 and 33 pN TGT are the shadows of the spherical cell body, not the close contacts. Scale bars, 10 µm. f Close-contact enclosed areas (n = 94, 95, 79, 75, 50 cells). g Ruptured TGT counts (n = 94, 96, 88, 81, 55 cells). Lines show mean ± SE. Two-sided t-test for p-values. Images are representative of multiple experiments and cell data points are combined from two or more independent experiments. Source data are provided as a Source Data file.
