Fig. 1: H3pT11 anti-correlates with H3K79me3.

a Correlation analyses of the ChIP-seq data of H3pT11 with other histone modifications in budding yeast. The correlation of each histone mark to itself along the diagonal is defined as 1.0, which is indicated in black line. b Distribution of H3pT11 and H3K79me3 across each gene through 1 kb upstream of the TSS to 1 kb downstream from the TES at all genes. Log₂ ratios of H3pT11 or H3K79me3 versus H3 at significantly enriched windows were used. Each row represents one gene. c Averaged metagene profiles of H3pT11/H3 and H3K79me3/H3 from a. d ChIP-seq tracks showing the enrichment of H3pT11/H3 and H3K79me3/H3 at representative genes. e Averaged distribution of H3pT11/H3 (log₂ (H3pT11/H3)) around the peaks of H3K79me3/H3. f Immunoblots of H3pT11 and H3K79me3 when cells were grown in glucose starvation conditions (SD-C). Cells were grown in YPD until OD₆₀₀ of 1.0. Cells were harvested, resuspended in SD-C and then grown for 0–6 h. g Immunoblots of Dot1 and Pyk1 in cells when grown in glucose starvation conditions (SD-C) for 6 h. h Immunoblot analysis of H3pT11 and H3K79me3 when cells were grown in YPD medium for 0–4 days. i Immunoblots of Dot1 and Pyk1 when cells were grown in YPD medium for 0–4 days. For f, h, data represent the mean ± SE; n = 3 biological independent experiments. Two-sided t-tests were used for statistical analysis. For g, i, a typical example of three biologically independent replicates was shown. Source data are provided with this paper.