Fig. 2: Loss of SESAME-catalyzed H3pT11 increases Dot1-catalyzed H3K79me3. | Nature Communications

Fig. 2: Loss of SESAME-catalyzed H3pT11 increases Dot1-catalyzed H3K79me3.

From: SESAME-catalyzed H3T11 phosphorylation inhibits Dot1-catalyzed H3K79me3 to regulate autophagy and telomere silencing

Fig. 2

a Immunoblot analysis of histone modifications and Dot1 in WT, H3T11A and H3T11D mutants when grown in YPD medium until OD600 of 1.0. b Immunoblot analysis of histone modifications and Dot1 in WT and pyk1-ts mutants when grown in YPD medium at non-permissive temperature (39 °C) for 2 h. c Distribution of H3K79me3 across each gene through 1 kb upstream of the TSS to 1 kb downstream from the TES at all genes in WT and H3T11A mutant. d Distribution of H3K79me3 across each gene through 1 kb upstream of the TSS to 1 kb downstream from the TES at all genes in WT and H3T11D mutant. e Averaged metagene profiles of H3K79me3/H3 in WT and H3T11A mutant. f Averaged metagene profiles of H3K79me3/H3 in WT and H3T11D mutant. g ChIP-seq tracks showing the enrichment of H3K79me3/H3 at representative genes in WT, H3T11A, and H3T11D mutants. For Fig. a, b, data represent the mean ± SE; n = 3 biologically independent experiments. Two-sided t-tests were used for statistical analysis. Source data are provided with this paper.

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