Fig. 3: Concentration-dependent kinetics of sDarken.
From: Next generation genetically encoded fluorescent sensors for serotonin
Responses of sDarken to increasing 5-HT concentrations, as indicated. a Patch fluorometry traces show averages of 6–8 sweeps. ON and OFF time constants were determined by single exponential fits (red lines). b The ON kinetics fasten with increasing 5-HT concentrations (top), the OFF kinetics are concentration independent (middle; mean τOFF indicated as dashed line). The signal changes are consistent with Kd ~100 nM (bottom). Number of patches given in parenthesis, means shown as crosses; statistical testing was performed using an ANOVA/Tukey–Kramer procedure, *p < 0.5, ***p < 0.001. For details on the experiment, analysis and model (inset) see Supplementary Note and Fig. S12.
