Fig. 2: Overall architecture of rA3GR8/E259A in complex with three types of AA-containing RNA.
From: Structural basis of sequence-specific RNA recognition by the antiviral factor APOBEC3G
To distinguish the two adenosines in the sequence motif 5′-AA-3′, we designated the 5′-end A as 5′A (in cyan) and the 3′-end A as 3′A (in green). a A schematic of the 5′-overhang AA dinucleotide dsRNA (RNA1-AAxtal). b The co-crystal structure of rA3GR8/E259A and RNA1-AAxtal as a dimer (PDB ID: 7UU5). c A 2Fo-Fc electron density map of the resolved RNA1-AAxtal contoured at 1 σ level. d A schematic of the 3′-overhang AA dinucleotide dsRNA (RNA2-AAxtal). e The co-crystal structure of rA3GR8/E259A and RNA2-AAxtal as a dimer (PDB ID: 7UU3). f A 2Fo-Fc electron density map of the resolved RNA2-AAxtal contoured at 1.5 σ level. g A schematic of the ssRNA (RNA3-AAxtal). h The co-crystal structure of rA3GR8/E259A and RNA3-AAxtal as a monomer (PDB ID: 7UU4). i A 2Fo-Fc electron density map of the resolved RNA3-AAxtal contoured at 1.5 σ level. In panels a, d, g, the RNA bases not marked in light gray are resolved in the structures. dsRNA8 represents an 8-bp double-stranded RNA molecule (Supplementary Table 1). The percentage of the buried RNA surface is shaded in light blue with the percentage value labeled. In panels b, e, and h, CD1 (NTD) is colored in pale green, CD2 (CTD) in pale cyan, and the CD1-CD2 linker 194RHL196 in wheat. See Supplementary Fig. 10 for additional views of the dimers shown in panels b and e.
