Table 1 Crystallographic data collection and refinement statistics
From: Structural basis of sequence-specific RNA recognition by the antiviral factor APOBEC3G
PDB ID | 7UU5 rA3G/RNA1-AAxtal (5′ Overhang dsRNA) | 7UU3 rA3G/RNA2-AAxtal (3′ Overhang dsRNA) | 7UU4 rA3G/RNA3-AAxtal (ssRNA with AA) | 8EDJ rA3G/RNA3-GAxtal (ssRNA with GA) |
|---|---|---|---|---|
Data collection | ||||
Space group | P1 | P1 | P212121 | P212121 |
Cell dimensions | ||||
a, b, c (Å) | 61.3, 71.6, 72.7 | 60.7, 67.2, 79.6 | 54.2, 68.0, 126.8 | 55.3, 68.1, 129.3 |
α, β, γ (°) | 119.4, 111.0, 90.1 | 98.3, 108.7, 112.7 | 90, 90, 90 | 90, 90, 90 |
Resolution (Å) | 46.5-2.90 | 45.7-3.10 | 46.4-2.10 | 40.7-1.83 |
(3.00-2.90)a | (3.21-3.10)a | (2.18-2.10)a | (1.90-1.83)a | |
Rmerge | 0.107 (0.503) | 0.130 (0.763) | 0.114 (0.621) | 0.085 (0.935) |
CC1/2 | 0.994 (0.845) | 0.990 (0.557) | 0.998 (0.922) | 0.999 (0.953) |
I/σI | 11.5 (1.6) | 8.9 (2.5) | 14.9 (3.1) | 21.0 (3.9) |
Completeness | 0.98 (0.92) | 0.98 (0.94) | 0.98 (0.87) | 1.00 (1.00) |
Redundancy | 3.3 (3.3) | 3.3 (2.4) | 12.9 (10.6) | 12.7 (13.2) |
Refinement | ||||
Resolution (Å) | 46.5-2.90 | 45.7-3.10 | 46.4-2.10 | 40.7-1.83 |
(3.00-2.90)a | (3.21-3.10)a | (2.18-2.10)a | (1.90-1.83)a | |
Rwork/Rfree | 0.189/0.241 | 0.170/0.210 | 0.184/0.205 | 0.169/0.192 |
No. of atoms | 6771 | 6627 | 3466 | 3566 |
Macromolecules | 6767 | 6623 | 3228 | 3229 |
Ligand/ion | 4 | 4 | 7 | 7 |
Water | 231 | 330 | ||
B-factor | ||||
Macromolecules | 86.9 | 94.3 | 45.2 | 37.3 |
Ligand/ion | 107.9 | 84.0 | 40.5 | 27.6 |
Water | 45.0 | 44.7 | ||
R. m. s. deviations | ||||
Bond lengths (Å) | 0.004 | 0.004 | 0.004 | 0.006 |
Bond angles (°) | 1.00 | 0.97 | 0.70 | 0.80 |
