Fig. 2: The Trpc1 or Trpc6 knockout inhibits TLR signaling pathway. | Nature Communications

Fig. 2: The Trpc1 or Trpc6 knockout inhibits TLR signaling pathway.

From: TRPC channels blockade abolishes endotoxemic cardiac dysfunction by hampering intracellular inflammation and Ca2+ leakage

Fig. 2: The Trpc1 or Trpc6 knockout inhibits TLR signaling pathway.The alternative text for this image may have been generated using AI.

a Pearson’s correlation test of all the detected genes of RNA-seq in the LPS-challenged mice compared to control. b Volcano plot of the changed genes in RNA-seq between Trpc1−/− and Trpc6−/− mice after LPS challenge. c Counting pie charts depicting the top-ranked biological process classification of the differentially expressed genes in RNA-seq using Gene Ontology terms. d The top 20 down-regulated pathways in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database of LPS-challenged Trpc1−/− or Trpc6−/− mice compared to the WT mice. e The heatmap of the genes in the TLR signaling pathway from the KEGG database based on RNA-seq analysis. The values from RNA-seq in a–e are obtained from 3 male mice per group. f Serum levels of TNF-α, IFN-β, IL-6, and IL-1β in mice 6 h after LPS challenge (mean ± SEM, n = 6 male mice samples per group). Statistical significance was determined using the one-way ANOVA with Tukey’s multiple comparisons test. TNF-α, exact P value = 2.4 × 10−12 (WT + LPS vs Trpc1−/−+LPS), 3.7 × 10−11 (WT + LPS vs Trpc6−/−+LPS); IFN-β, exact P value = 9.9 × 10−13 (WT + LPS vs Trpc1−/−+LPS), 3.1 × 10−12 (WT + LPS vs Trpc6−/−+LPS); IL-6, exact P value = 5.4 × 10−11 (WT + LPS vs Trpc1−/−+LPS), 8.3 × 10−13 (WT + LPS vs Trpc6−/−+LPS); IL-1β, exact P value = 8.0 × 10−8 (WT + LPS vs Trpc1−/−+LPS), 3.4 × 10−5 (WT + LPS vs Trpc6−/−+LPS). g Representative photomicrographs (left panel) and quantitative data (right panel) of MIP-1α immunohistochemical staining on ventricular tissues. Arrows show MIP-1α positive cells (mean ± SEM, n = 12 images from 4 male mice per group). Statistical significance was determined using the one-way ANOVA with Tukey’s multiple comparisons test. Source data are provided as a Source Data file.

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