Fig. 3: TRPC1 and TRPC6 associate with TLR4 and Ca²⁺ signaling pathways.

a-b The effects of Trpc1 or Trpc6 knockout on nuclear factor-κB (NF-κB) and mitogen-activated protein kinases (MAPK) signaling pathways in the hearts of mice 4 h after LPS challenge (pooled tissues from 3 male mice per sample, n = 3 biological independent experiments). c The effects of Trpc1 or Trpc6 knockout on Toll like receptor 4 (TLR4)‐mediated myeloid differentiation primary response protein 88 (MyD88)- and TIR domain-containing adaptor inducing IFN-β (TRIF)-dependent signaling pathways in the hearts of mice 4 h after LPS challenge (pooled tissues from 3 male mice per sample, n = 3 biological independent experiments). d Heatmap depicting the genes involved in the Ca²⁺ signaling pathway from the KEGG pathway database based on RNA-seq analysis (n = 3 male mice per group). e-f The effects of Trpc1 or Trpc6 deletion on Calm2 mRNA (n = 3 male mice with triplicate measurements taken, mean ± SEM) and calmodulin (CaM) protein expressions (pooled tissues from 3 male mice per sample, n = 3 biological independent experiments) in the hearts of mice 4 h after LPS challenge. g-h, The activity of calcineurin (mean ± SEM, n = 6 male mice samples per group) and NFAT3 nuclear translocation (pooled tissues from 3 male mice per sample, n = 3 biological independent experiments) in the hearts of mice 4 h after LPS challenge. Statistical significance was determined using the one-way ANOVA with Tukey’s multiple comparisons test. Source data are provided as a Source Data file.