Fig. 9: SKF cures septic cardiac dysfunction. | Nature Communications

Fig. 9: SKF cures septic cardiac dysfunction.

From: TRPC channels blockade abolishes endotoxemic cardiac dysfunction by hampering intracellular inflammation and Ca2+ leakage

Fig. 9: SKF cures septic cardiac dysfunction.The alternative text for this image may have been generated using AI.

a Representative M-mode echocardiography still and the statistical analysis of ejection fraction in vehicle- or SKF-treated mice at 6 h after LPS challenge (mean ± SEM, n = 6 male mice per group). Statistical significance was determined using the one-way ANOVA with Tukey’s multiple comparisons test. Exact P value = 4.5 × 10−7 (LPS vs LPS + SKF 10 mg/kg) and 2.9 × 10−10 (LPS vs LPS + SKF 20 mg/kg). b SKF treatment on the serum levels of TNF-α and IFN-β in LPS-challenged mice (mean ± SEM, n = 6 male mice samples per group). Statistical significance was determined using the one-way ANOVA with Tukey’s multiple comparisons test. TNF-α, exact P value = 2.0 × 10−6 (LPS vs LPS + SKF 10 mg/kg) and 1.2 × 10−9 (LPS vs LPS + SKF 20 mg/kg); IFN-β, exact P value = 8.7 × 10−5 (LPS vs LPS + SKF 5 mg/kg), 1.0 × 10−12 (LPS vs LPS + SKF 10 mg/kg), and 8.3 × 10−13 (LPS vs LPS + SKF 20 mg/kg). c SKF treatment on the serum markers of myocardial damage in LPS-challenged mice (mean ± SEM, n = 6 male mice samples per group). Statistical significance was determined using the one-way ANOVA with Tukey’s multiple comparisons test. Troponin-I, exact P value = 7.0 × 10−6 (LPS vs LPS + SKF 20 mg/kg); Troponin-T, exact P value = 1.0 × 10−6 (LPS vs LPS + SKF 20 mg/kg). d Kaplan-Meier survival curves of single-dose SKF treatment (n = 10 male mice per group). e Survival curves of 10 mg/kg SKF multiple-dosing (every 12 h, n = 10 male mice per group). Statistical significances in d and e were determined using the log-rank test. Exact P value = 3.0 × 10−6 (SKF vs LPS). f Echocardiographic assessment of left ventricular function of SKF-treated mice at 6 h after CLP surgery. Typical heart M-mode echocardiography still (left), ejection fraction (right panel) are shown (mean ± SEM, n = 6 male mice per group). Statistical significance was determined using the one-way ANOVA with Game Howell’s multiple comparisons test. Exact P value = 5.7 × 10−5 (WT vs CLP) and 7.0 × 10−6 (CLP vs CLP + SKF). g The serum levels of TNF-α and IFN-β in the SKF-treated CLP mice (mean ± SEM, n = 6 male mice samples per group). Statistical significance was determined using the one-way ANOVA with Tukey’s multiple comparisons test. TNF-α, exact P value = 5.8 × 10−9 (WT vs CLP) and 1.2 × 10−5 (CLP vs CLP + SKF); IFN-β, exact P value = 5.8 × 10−9 (WT vs CLP) and 2.3 × 10−7 (CLP vs CLP + SKF). h SKF treatment on the serum markers of myocardial damage in CLP mice (mean ± SEM, n = 6 male mice samples per group). Statistical significance was determined using the one-way ANOVA with Tukey’s multiple comparisons test. Troponin-I, exact P value = 5.8 × 10−9 (WT vs CLP) and 1.3 × 10−7 (CLP vs CLP + SKF); Troponin-T, exact P value = 5.8 × 10−9 (WT vs CLP) and 2.5 × 10−8 (CLP vs CLP + SKF); Creatine kinase-MB, exact P value = 5.8 × 10−9 (WT vs CLP) and 2.2 × 10−7 (CLP vs CLP + SKF). i Kaplan-Meier survival curves of multiple-dosing SKF treatment on CLP mice (n = 10 male mice per group). Statistical significance was determined using the log-rank test. Exact P value = 3.0 × 10−6 (WT vs CLP). Source data are provided as a Source Data file.

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