Fig. 5: SARS-CoV-2 infection exhibits higher replicative competence in COPD bronchial organoids. | Nature Communications

Fig. 5: SARS-CoV-2 infection exhibits higher replicative competence in COPD bronchial organoids.

From: The establishment of COPD organoids to study host-pathogen interaction reveals enhanced viral fitness of SARS-CoV-2 in bronchi

Fig. 5: SARS-CoV-2 infection exhibits higher replicative competence in COPD bronchial organoids.The alternative text for this image may have been generated using AI.

a–d Viral replication kinetics from L-WU-infected and G-614G infected NPOs and BOs at 1, 24, 48, 72 and 96 hpi by TCID50 assay. n = 3 biologically independent experiments for NPO and BO infections are illustrated. Data are presented as means ± SEM and unpaired t-test is performed. *P < 0.05; ***P < 0.001. [NPO L-WU: 24 hpi=0.0494; 72 hpi=0.0004; BO L-WU: 24 hpi=0.0110; 96 hpi=0.0152; BO G-614G: 24 hpi=0.0294; 96 hpi=0.0457]. e–h qRT-PCR analysis of total RNA extracted from L-WU-infected and G-614G -infected NPOs and BOs derived from non-diseased individuals and COPD patients respectively at 72 hpi for IL-6, IFN-β and CXCL10. n = 3 biologically independent experiments are illustrated. Data are presented as means ± SEM and an unpaired t-test is performed. *P < 0.05. [Fig. 5h: IL-6 = 0.0237; IFN-β = 0.0248] Source data are provided as a Source Data file.

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