Fig. 1: spd_1643-1642 encodes an ET uptake transporter in S. pneumoniae denoted EgtU.
From: Discovery and structure of a widespread bacterial ABC transporter specific for ergothioneine
a Hypothesized functional roles of the genes of the streptococcal conserved operon30 harboring a candidate QAC ABC transporter, encoded by spd_1642-1643. b Structure of HPE-IAM-derivatized (capped) ET and LC traces of capped ET from authentic ET (standard) and cell lysates from the indicated S. pneumoniae D39 strains grown on BHI media. WT, wild-type; ∆egtU, markerless deletion of most of the egtU gene, which was then repaired via insertion of a wild-type egtU allele (∆egtU repaired). c MS1 (left) and LC-MS/MS (right) spectra of HPE-IAM capped ET found in a wild-type (WT) cell lysate from cells grown in BHI vs. authentic ET (standard). d Normalized content of ET, GSH and CYS found in the indicated strains of S. pneumoniae D39 grown in BHI in biological triplicate. *, not detected (≤0.0001 nmol thiol/mg protein). e Normalized content of ET and CYS in the indicated strains of S. pneumoniae D39 grown in CDM supplemented with indicated concentration of ET in biological triplicate. *, not detected (≤0.001 nmol/mg protein). GSH is not detected in these cell lysates (≤0.0001 nmol/mg protein). Molar thiol concentrations estimated from nmol/mg protein in S. pneumoniae as described88 (see also Supplementary Fig. 5). f Same as d but with the indicated egtUA mutant strains. g ATPase activity of WT vs. mutant EgtUA proteins in vitro. **p < 0.01, ***p < 0.001 in a one-sided t-test. Data for d, e, f, and g are shown as the mean and standard deviation of three independent replicates, with individual measurements shown as red circles. Source data for d, e, f and g are provided as a Source Data file.
