Fig. 6: EgtU homologs cluster in a grouping of highly similar sequences within a subcluster of SSN cluster 2.
From: Discovery and structure of a widespread bacterial ABC transporter specific for ergothioneine
a Several EgtU sequence metanodes are highlighted with a large yellow circle, including L. monocytogenes BilEB and E. faecalis EgtUC biochemically characterized here. The lower (SpEgtU-like), middle, left and upper subclusters of SSN cluster 2 are labeled, with full sequence conservation maps shown in Supplementary Fig. 19. The middle subcluster sequences are derived from anaerobes or obligate anaerobes, including those recently studied in C. difficile57. b Representation of SSN cluster 2 colored according to neighborhood connectivity (NC; see scale), with those sequences within a single metanode that are most closely related characterized by a large NC index (and shaded magenta). c Sequence logo representation of conservation in the SBD of EgtUs of those closely related sequences encircled by the red box in b. Secondary structure of the SBD is indicated, based on the structure of SpEgtUC. Residues discussed are highlighted with a specific symbol of interaction, as indicated. See text for additional details. TMD, predicted sites of interaction with the transmembrane domain. Source data for these images shown in panels a, b are provided as a Source Data file.
