Fig. 5: YL-939 binds to the PHB2 protein.
From: Non-classical ferroptosis inhibition by a small molecule targeting PHB2
A SPR analysis of YL-939 (left) or YL-447 (right) binding to the recombinant PHB21–194 protein. Data were obtained from two biological replicates. B DSF analysis of YL-939 or YL-447 binding to the recombinant PHB21–194 protein. Data were obtained from three biological replicates. C A predicted binding mode of YL-939 with PHB21–194. The three-dimensional structure of PHB2 predicted by AlphaFold (https://alphafold.ebi.ac.uk/entry/Q99623) was used as the receptor structure. D DSF analysis of YL-939 binding to the recombinant mutated PHB21-194-D82A protein. Data were obtained from three biological replicates. E DSF analysis of YL-939 binding to the recombinant mutated PHB21-194-D127A protein. Data were obtained from three biological replicates. F Protein expression of each recombinant PHB2. Blots shown are representative of two biological replicates. G Effects of PHB2 mutants or wild type on ferroptosis protection of YL-939 treatment and sensitivity of cells to ferroptosis. Data represent the mean ± SD of three biological replicates. Statistical analyses were performed by two-way ANOVA with Sidak’s multiple comparisons test: **p < 0.01. Source data are provided as a Source data file.
