Fig. 6: PHB2 participates in the regulation of ferroptosis and mechanism of action.
From: Non-classical ferroptosis inhibition by a small molecule targeting PHB2
A Knockdown of PHB2 using siRNA abolished erastin-induced cell death. Western Blot analysis of PHB2 protein expression is shown on the left. Blots shown are representative of two biological replicates. ES-2 cells transient transfected siRNA were treated with Erastin (10 μM) for 10 h. Representative images of cell state observed under the microscope are shown on the right. Scale bars: 5 μm. B PHB2 expression affects the sensitivity of cells to ferroptosis. Blots shown are representative of two biological replicates. Data represent the mean ± SD of three biological replicates. Statistical analyses were performed by two-way ANOVA with Sidak’s multiple comparisons test: **p < 0.01. C Inhibition of PHB2 by shRNA or YL-939 increased the expression level of ferritin protein. Blots shown are representative of three biological replicates. D Effects of PHB2 knockdown on ferroptosis key genes. Data represent the mean ± SD of three biological independent experiments. Statistical analyses were performed by two-way ANOVA with Sidak’s multiple comparisons test. Specific p-values are indicated in the figure. E PHB2 knockdown decreased the iron content determined by Iron assay kit. Data represent the mean ± SD of three biological replicates. Statistical analyses were performed by two-tailed unpaired Student’s t-test. Specific p-values are indicated in the figure. F PHB2 knockdown decreased the iron content examined with fluorescent probes. Cells were stained with Fe2+ probe (red) and Dapi (blue). Representative images of two biological replicates are shown. Scale bars: 10 μm. Source data are provided as a Source data file.
