Fig. 4: miR-1304-3p induced adipocytes promote cancer cell growth.

a Cancer cells were labeled with mCherry and adipocytes were labeled with GFP. After differentiation of adipocytes by adipogenesis inducers including IBMX and rosiglitazone and changed to serum-free medium, cancer cells were seeded directly on top of adipocytes and cultured for 48 hours. b Pictures showing endpoint of cancer-adipocyte co-culture. Number of cancer cells per field (N = 6 each) was counted with ImageJ. Unpaired two-tailed student t test, p = 0.000009. Scale bar = 100 μm. c Tumor xenograft cryosections were stained with Ki67 by IHC, and percentage of Ki67-positive cells were analyzed. n = 5 biologically independent animals. Unpaired two-tailed student t test was used (p = 0.0002). Scale bar = 100 μm. d Lipids extracted from condition medium of SGBS-ctr and SGBS-1304 adipocytes were analyzed by mass spectrometry and normalized peak areas for each class of lipid molecules were shown. Unpaired two-tailed student t test was performed. P = 0.0273 (Cer), 0.5863(ChE), 0.0015(Co), 0.3043(DG), 0.8412(LPC), 0.9260(LPE), 0.0356(PC), 0.1635(PE), 0.0784(PS), 0.0711(SM), <0.000001(TG), n = 3 independent experiments. e Top 20 differentially secreted lipid molecules were shown. Unpaired two-tailed student t test was performed to calculate the p value. Data are presented as mean values +/- SEM.