Fig. 1: Working principle of MAI-SIM. | Nature Communications

Fig. 1: Working principle of MAI-SIM.

From: Machine learning assisted interferometric structured illumination microscopy for dynamic biological imaging

Fig. 1: Working principle of MAI-SIM.The alternative text for this image may have been generated using AI.

A Schematic of the interferometric design. Three small mirror pairs, each forming a Michelson interferometer, are tilted to form interference patterns at angles, θ1–3, separated by 2π/3. Large steps of the scanning mirror change the mirror pair addressed by the incoming beam (dashed line) and hence the rotation of the fringe pattern. Small steps of the scanning mirror laterally displace the position of the interference fringes and cause a phase shift, δϕ, in the excitation pattern. Different wavelengths (blue and green patterns) produce fringes with different spacing and hence an unequal phase shift. B Schematic of detection optics. Using several dichroic mirrors and emission filters, the three-way image splitter allows for the simultaneous display of up to three color channels on the camera chip. For full details on the optical setup and a rendering of the layout, see Supplementary Fig. 1.

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