Fig. 3: The effect of NAM supplementation on mitochondrial characteristics of 1% protein-fed model. | Nature Communications

Fig. 3: The effect of NAM supplementation on mitochondrial characteristics of 1% protein-fed model.

From: The role of the tryptophan-NAD + pathway in a mouse model of severe malnutrition induced liver dysfunction

Fig. 3: The effect of NAM supplementation on mitochondrial characteristics of 1% protein-fed model.The alternative text for this image may have been generated using AI.

a Representative immunofluorescence images of mitochondrial (×60 magnification). HSP60 was used to stain mitochondrial in red, and DAPI was used to counter stain nucleus in blue (n = 3 replicates). b Relative mtDNA copy number (mtDNA/beta-globin) (n = 6 per group). c, d Representative western blots and quantification of HSP60 and TOM20 (n = 3 per group). e ATP levels (n = 11 for 18 and 1%; n = 7 for 1%+NAM). f, g Representative western blots and quantification of complex I, complex IV and complex V (n = 3 per group). h Relative expression of β-oxidation genes compared to control (n = 8 for 18%, n = 8 for 1%, and n = 9 for 1%+NAM). i Relative expression of lipid genesis genes compared to control (n = 9 for 18%; n = 8 for 1 and 1%+NAM). j Time series of oxygen consumption rate (OCR) measurements by Seahorse XFe96 analyzer. k Quantification of OCR (n = 4 per group). One-way ANOVA followed by Tukey’s post hoc test. Unless stated differently in the figure legend, all n represents biologically independent samples. Data are shown as the mean ± SEM. Scale bars are as indicated.

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