Fig. 3: STED-ISM imaging.

In (a), we compare raw images of fluorescent beads with the corresponding ISM reconstructions. In (b), we show the shift vectors and fingerprint calculated from an image of the beads. In (c), we show the resolution gain (left) and the signal gain (right) of STED-ISM with respect of STED, for increasing STED powers. We measure the resolution and the signal, respectively, as the FWHM and the peak value of the successful fit of the fluorescent bead to a Gaussian curve. The graphs report the average values of multiple beads with the corresponding standard errors. In (d), we show detailed regions of images of tubulin-labeled fixed cells. In (e), we show detailed images of living Hela cells with SIR tubulin labeling. More specifically, we compare raw STED (left), STED-ISM (right, upper corner) and the result of multi-image deconvolution STED-ISM⁺ (right, bottom corner). All results are shown with increasing STED power, from left to right. The full images are shown in Supplementary Figs. 3, 4. The values in white are the images' resolution, estimated using a fit to a Gaussian model (a) or Fourier ring correlation (d and e).