Fig. 6: Volumetric focus-ISM.
From: Focus image scanning microscopy for sharp and gentle super-resolved microscopy
In (a), we show the STED image of a tubulin-labelled fixed Hela cell (left). The f2-ISM reconstruction (right) show negligible loss of in-focus photons and greatly enhanced contrast, obtained by removing the out-of-focus content. In (b), we show the confocal image of the same cell (left). Even in this case, the f2-ISM reconstruction (right) shows negligible loss of in-focus photons and greatly enhanced contrast. The axial cross-sections are obtained from the x-axis highlighted with a white dashed line.
