Fig. 3: Adissp is required for iWAT browning and energy homeostasis.

a Representative images of Ucp1 immunofluorescence staining in iWAT from 3-month-old male Adissp adipose-specific knockout (ADKO) mice and Flox controls housed at room temperature and after 8 h cold exposure (n = 3 mice per group). Scale bar, 200 μm. b Gene expression analysis in iWAT from 5-month-old female Adissp ADKO mice (n = 8) and Flox controls (n = 9) after 7 h cold exposure. c Rectal temperature of mice from (b) during cold exposure. d Quantification of ³H-2DG uptake in indicated tissues from female Adissp ADKO mice (n = 5) and Flox controls (n = 6) after 3 h cold exposure. e Gene expression analysis in iWAT from 2 days CL-316,243 (1 mg/kg body weight) administrated 3-month-old female mice (n = 12 mice per group). f Body weight of male Adissp ADKO mice (n = 15) and Flox controls (n = 12) on HFD. g, h Representative images of H&E staining in BAT and iWAT (g) and Ucp1 immunofluorescence staining in iWAT (h) from mice in (f) on 21 weeks of HFD (n = 3 mice per group). Scale bar, 200 μm. i Fat mass and lean mass of a second cohort of mice after 16 weeks of HFD feeding (n = 5 mice per group). j, GTT in mice from (f) on 18 weeks of HFD (Flox, n = 7; ADKO, n = 8). k Insulin tolerant test (ITT) in mice from (f) on 19 weeks of HFD (Flox, n = 7; ADKO, n = 8). Data are mean ± s.e.m. P values were determined by two-tailed Student’s t test (b, d, e, i and AUC in j and k) and two-way repeated measures ANOVA with post hoc test by Fisher’s LSD test (c, f, j, and k), n.s. (not significant). Source data are provided as a Source Data file.