Fig. 4: Endocrine action of Adissp.
From: A brown fat-enriched adipokine Adissp controls adipose thermogenesis and glucose homeostasis
a Detection of circulating ADISSP protein (n = 3 mice per group). b Representative images of H&E staining and Ucp1 immunofluorescence staining in iWAT from indicated adenovirus-injected mice (n = 3 mice per group). Scale bar, 200 μm. c mRNA expression of indicated genes in iWAT from adenovirus-injected mice (GFP, n = 5; Adissp, n = 6). d GTT in adenovirus-injected mice fasted for 5 h (GFP, n = 5; Adissp, n = 6). e Quantification of 3H-2DG uptake in indicated tissues from adenovirus-injected mice (GFP, n = 5; Adissp, n = 6) after 5 h fasting. f GTT in HFD-induced obese mice that were fasted overnight (GFP, n = 5; Adissp, n = 6). g Basal glucose of mice in (f) that were fasted for 5 h (GFP, n = 5; Adissp, n = 6). h Gene expression in iWAT from 3-month-old WT male mice intravenously injected with Adissp protein or vehicle once a day for 9 days (Vehicle, n = 10; Adissp, n = 9). i Representative images of Ucp1 immunofluorescence staining in iWAT from mice in (h) (n = 7 mice per group). Scale bar, 200 μm. j GTT in another cohort of 3-month-old WT male mice at day 7 of injection (n = 7 mice per group). Data are mean ± s.e.m. P values were determined by two-tailed Student’s t test (c, e, g, h and AUC in d, f, and j) and two-way repeated measures ANOVA with post hoc test by Fisher’s LSD test (d, f, and j), n.s. (not significant). Source data are provided as a Source Data file.
