Fig. 3: Effect with activation of MRN serotonergic neurons on reward consumption.
a Schematic representation of AAV injection and fiber implantation. b Two-bottle choice test. The position of the bottle paired with light was counter-balanced between days 3 and 4. c Total intake of sucrose solution in each bottle. Two-way repeated-measures ANOVA with Sidak post hoc test, virus × light interaction F1,13 = 26.97, P = 0.0002, virus F1,13 = 0.09712, P = 0.7603, light F1,13 = 26.01, P = 0.0002; post hoc test: Valued condition OFF-CheRiff vs ON-CheRiff ***P < 0.001, n = 8 (Venus) and 7 (CheRiff) mice). d Total intake of solution from both of bottles. Two-tailed unpaired t-test with Welch’s correction, t7.378 = 0.2949, P = 0.7762, n = 8 (Venus) and 7 (CheRiff) mice. e Schematic representation of AAV injection and fiber implantation and distance from the bregma. f Head-fixed setup. Mice were secured by head clamps attached to the chamber frame. A 10% sucrose reward was delivered through the needle. g Schematic diagram of the optogenetic stimulation. A 2 μL drop of 10% sucrose solution reward was delivered every 10 s. h, i Number of licks during each phase of the fixed-time schedule task (Black: Pre, Red: Stim, Gray: Post). Each time point of the graph represents the mean number of licks for 2 s. Three-way ANOVA for repeated measures (Phase effect (Venus) (n = 6 mice, F2,18 = 0.22, P = 0.81), Phase effect (CheRiff) (n = 10 mice, F2,18 = 7.82, P = 0.0036), Virus × Phase interaction (F2,28 = 3.36, P = 0.049), Virus × Time (−6 to 2 s) interaction (F4,56 = 8.29, P = 2.52 × 10−5)). Multiple comparisons for phase effect in the CheRiff group (Pre vs Post (P = 0.012), Pre vs Stim (P = 0.016), Stim vs Post (P = 0.27) phase effects. Data are presented as mean ± s.e.m. Error bars indicate s.e.m. Source data are provided as a Source Data file. An illustration of a mouse was obtained from Pixabay.
