Fig. 2: Fast-bright tracing the auditory cortex projection pathways with H129Amp tracer system.
a Schema of the simplified projection pathways of the auditory cortex. AC auditory cortex, Cont. AC contralateral AC, MG medial geniculate nucleus, LA lateral amygdala, GPe external globus pallidus, LC locus coeruleus. b Tracing AC outputs with H129Amp tracer system in wild-type C57BL/6 mice. The H129Amp- tracer system (H129Amp-CTG 1.5 × 108 pfu/ml and helper 1.5 × 108 pfu/ml, in 300 nl) was injected into the AC (AP: −2.80 mm; ML: −4.13 mm; DV: −2.38 mm) of wild-type C57BL/6 mice. The brains were collected at 1 and 5 days post-injection (Day 1 and Day 5), and images were obtained after cryosection and DAPI counterstaining. c Labeled neurons in the injection site at Day 1. Representative images of the injection site AC at Day 1 are shown (left panel), and the boxed region is displayed with higher magnification (middle panel). The numbers of AC neurons doubled-labeled by tdT/GFP (Yellow, 588 ± 103), or single-labeled by GFP (Green, 352 ± 95) or tdT (red, 210 ± 56) were quantified and presented as means ± SEM from three mice (right panel). d–i Representative tracing results at Day 5. Representative images of the injection site AC (d), and representative AC-innervating regions (e–i) are shown. Images with higher magnifications of the boxed areas are presented in the right panels. Potential starter neurons labeled by both GFP and tdTomato (merged as yellow) are indicated with white arrowheads. A representative GFP-labeled neuron in Cont. AC is further magnified and the morphological details are displayed (h1 and h2). Source data are provided as a Source Data file.
