Fig. 3: Eliglustat reduces bone loss in a diet-induced obesity MGUS model.

a Schematic overview and experiment design. Four-week-old female C57BL/6J mice were divided into MM injection with normal diet (MM, n = 4 biologically independent animals), HFD with MM (HFD + MM, n = 10 biologically independent animals), and HFD with MM plus eliglustat (HFD + MM + Elig, n = 10 biologically independent animals). b Body weight (g) was measured over the duration of the experiment. Statistical analysis was performed by comparing with MM group (MM, n = 4; HFD + MM, n = 10, and HFD + MM + Elig, n = 10 biologically independent animals). c Representative micro-CT reconstruction images of tibiae trabecular bones from respective groups. d–k Micro-CT analysis parameters of tibiae: BV/TV, BS, Tb.N, Conn.D, Tb.Th, BS/BV, Tb.Sp, and trabecular BMD (MM, n = 4; HFD + MM, n = 10, and HFD + MM + Elig, n = 10 biologically independent animals). l, m Osmium tetroxide staining to detect regulated BMAT (in red box) by micro-CT in MM, HFD + MM, and HFD + MM + Elig groups (MM, n = 4; HFD + MM, n = 10, and HFD + MM + Elig, n = 10 biologically independent animals). n–q Representative TRAP/0.2% methyl green stained tibial histological sections showing red OCs (n). The result is representative of two independent experiments. Bone histomorphometry parameters including Oc.S/BS (o), N.Oc/T.Ar (p), and N.Oc/B.Pm (q) (MM, n = 4; HFD + MM, n = 10, and HFD + MM + Elig, n = 10 biologically independent animals). Data are presented as mean values ± SEM. Exact p-values are depicted in the figure. Statistical analysis was performed using One-way ANOVA. Source data are provided as a Source Data file.