Table 1 Buffer compositions used in experiments on cultured ND7/23 cells

From: Calcium-permeable channelrhodopsins for the photocontrol of calcium signalling

	Concentration in mM
	NaCl	KCl	CsCl	CaCl₂	MgCl₂	HEPES/Tris	EDTA	NMGCl	Osmolarity [mOsm]	pH	Figure reference
High [CaCl₂]_e	1	1	1	70	2	10	0	0	320	7.2	Figs. 1, 2, S2–S4, S6–S8
High [NaCl]_i	110	1	1	2	2	10	10	0	290	7.2	Figs. 2, S2–S4, S6–S8, S14
High [NaCl]_e	140	1	1	2	2	10	0	0	320	7.2	Figs. 2, S2–S4, S6–S9, S13
High [NMGCl]_e	1	1	1	2	2	10	0	140	320	7.2	Figs. 2, S2–S4, S6–S8
High [CaCl₂]_e	1	1	1	70	2	10 (Tris)	0	0	320	9	Figs. 2, S2–S4, S6–S8
High [MgCl₂]_e	1	1	1	2	70	10	0	0	320	7.2	Figs. 2, S9, 14
DVCF: high [NaCl]_i	110	1	1	0	0	10	0.05	0	290	7.2	Figs. 3, 4, S10–S13, S15, S16
144 mM [NaCl]_e, 0 mM [CaCl₂]_e	144	1	1	0	0	10	0	0	320	7.2	Figs. 3, 4, S10–S13, S15, S16
140 mM [NaCl]_e, 2 mM [CaCl₂]_e	140	1	1	2	0	10	0	0	320	7.2	Figs. 3, 4, S12, 15
134 mM [NaCl]_e, 5 mM [CaCl₂]_e	134	1	1	5	0	10	0	0	320	7.2
124 mM [NaCl]_e, 10 mM [CaCl₂]_e	124	1	1	10	0	10	0	0	320	7.2
0 mM [NaCl]_e, 70 mM [CaCl₂]_e	1	1	1	70	0	10	0	0	320	7.2	Figs. 3, S10, 12, 14
142 mM [NaCl]_e, 1 mM [CaCl₂]_e	142	1	1	1	0	10	0	0	320	7.2	Figs. 4, S15
143 mM [NaCl]_e, 0.5 mM [CaCl₂]_e	143	1	1	0.5	0	10	0	0	320	7.2
143.8 mM [NaCl]_e, 0.1 mM [CaCl₂]_e	144	1	1	0.1	0	10	0	0	320	7.2
104 mM [NaCl]_e, 20 mM [CaCl₂]_e	104	1	1	20	0	10	0	0	320	7.2
DVCF: 144 mM [NMGCl]_e, 0 mM [CaCl2]_e	1	1	1	0	0	10	0	144	320	7.2	S12, 13
140 mM [NMGCl]_e, 2 mM [CaCl₂]_e	1	1	1	2	0	10	0	140	320	7.2	S12
134 mM [NMGCl]_e, 5 mM [CaCl₂]_e	1	1	1	5	0	10	0	134	320	7.2
124 mM [NMGCl]_e, 10 mM [CaCl₂]_e	1	1	1	10	0	10	0	124	320	7.2
	NaCl	KCl	CsCl	CaCl₂	MgCl₂	HEPES	TEA	NMGCl
High [KCl]_e	1	140	5	2	2	10	20	0	320	7.2	Figs. 2, S9
	NaCl	KCl	CsCl	BaCl₂	SrCl₂	HEPES	MgCl₂	NMGCl			Figs. 2, S9
144 mM [NaCl]_e, 2 mM [MgCl₂]_e	144	1	1	0	0	10	2	0	320	7.2	S13, S14
144 mM [NaCl]_e, 2 mM [BaCl₂]_e	144	1	1	2	0	10	0	0	320	7.2	S13
144 mM [NaCl]_e, 2 mM [SrCl₂]_e	144	1	1	0	2	10	0	0	320	7.2	S13
	NaCl	KCl	CsCl	CaCl₂	MgCl₂	HEPES/Tris	EDTA	NMGCl			S13
140 mM [NaCl]_e, 2 mM [MgCl₂]_e	140	4	0	2	2	10	0	0	320	7.2	4A–C
144 mM [NaCl]_e, 0 mM [MgCl₂]_e	144	4	0	2	0	10	0	0	320	7.2	4A–C

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Table 1 Buffer compositions used in experiments on cultured ND7/23 cells

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