Fig. 6: Dynamics of condensation of BRD4-NUT and p300.

a Example image of nuclear droplets. Red square: Time laps imaging of BRD4-NUT and p300 droplets; Scale bar, 4 μm. b Time laps imaging after addition of 3% [w/v] 1,6 Hexanediol. Scale bar, 12 μm. Experiments in a-b were repeated independently two times with consistency. c Top: Example image of droplets (red square) after photobleaching. Scale bar, 2 μm. Bottom: Quantification of the FRAP experiments. N = 3 independent technical replicates were done. Averages and standard error of mean (SEM; grey bars) are shown. d Quantification of the characteristic time of recovery rates for different GFP-BRD4-NUT mutants (green, top) and RFP-p300 (red, bottom). N = 3–4 technical replicate FRAP experiments were done for each mutant (40 to 65 foci measured). Averages recovery rates for each N replicate experiment are shown as dots (•). Bar height represents the mean recovery rate value. Statistical differences in recovery rates were determined by a two-sided Student’s t-test. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; n.s. non-significant difference as compared to WT. e Half-bleach FRAP experiment. Top: The area before (left) and after (right) laser bleach are shown. The red box indicates the bleached area. Scale bar, 0.5 μm. Bottom: The change in fluorescence intensity along the A-B axis was analysed in the Kymograph. f Top: Plot of fluorescence recovery from the Kymograph for the unbleached area (orange), centre (blue) and periphery (grey) of the droplet. Bottom: cartoon of the internal and external exchange. Experiments in e, f were repeated twice with consistency.