Fig. 6: Differential gene expression analysis of HD and control OPCs and OLs.

Venn diagram analysis of the DEGs in OPCs (a) and OLs (b). The number of DEGs that are increased (black) or decreased (red) in HD nuclei is highlighted per overlap sector. The stars indicate the DEGs that are shared across all regions, and the # indicates the DEGs shared between the Cingulate and Accumbens. c Gene ontology (GO) term analysis of differentially expressed genes in select sectors of the Venn diagrams HD versus control OLs and OPC (from panels a, b). The * and # signs correspond to the DEGs shared across all regions and DEGs shared between accumbens and cingulate OL and OPCs, respectively (purple = OPC DEGs, and green = OL DEGs). The sign of the negative log10 of the adjusted p value indicates the direction of changes; positive sign corresponds to genes increased in HD, and negative sign corresponds to genes decreased in HD. Heat shock protein encoding genes HSPA1A, HSPH1, HSPA4L, HSP90AA1, HSPB1, HSPA4, HSPD1, HSPA1A, HSPA1B, and HSPB1. d Scatter plot of the correlation coefficients of genes that correlate with CAG repeats in OPCs (y-axis) and OLs (x-axis). The graph plots the regression coefficients of each gene in OLs versus OPCs; the upper right quadrant represents genes with positive correlations in both OPCs and OL, the lower left quadrant genes that have negative correlations in both. The color of the genes correspond to whether the coefficient was significant in OLs only (green), OPCs and OLs (blue), or OPCs only (purple). e KEGG and Reactome pathway enrichment analysis of the genes that significantly correlate with CAG repeats in OPCs and OLs (top panel), OLs (middle panel), or OPCs (lower panel). The negative log10 of the adjusted p value is indicated on the x-axis, and the pathways on the y-axis. The color of each circle corresponds to the percentage of overlap between the CAG-correlated genes and the genes in each pathway.