Fig. 2: Sirt6 regulates cartilage development and embryonic chondrocyte senescence.
From: Sirt6 attenuates chondrocyte senescence and osteoarthritis progression
a–c Gross appearance of Sirt6flox/flox and Sirt6 cKO (a), double staining with alizarin red and alcian blue of the whole skeleton of Sirt6flox/flox and Sirt6 cKO littermate embryos (E16.5, E18.5, and P0) (b) and length of long bones and vertebra (first to fifth lumbar spines) of Sirt6flox/flox and Sirt6 cKO littermate embryos (E18.5) (c). n = 6 mice per group. d Masson trichrome staining of whole tibias. The percentage of the length of the bone area, hypertrophic zone, and proliferative zone over the total tibia length of Sirt6flox/flox and Sirt6 cKO littermate embryos (E16.5, E18.5, and P0). n = 6 mice per group. e Representative immunohistochemistry of Col II, Aggrecan, and Col X in the tibia of the Sirt6flox/flox and Sirt6 cKO mice and quantitative analysis. n = 6 mice per group. f TUNEL assays in tibia sections of Sirt6flox/flox and Sirt6 cKO mice at E16.5 and quantitative analysis. n = 6 mice per group. g, h Representative images of immunostaining of P21 and P53 in tibias of Sirt6flox/flox and Sirt6 cKO mice and quantitative analysis (E13.5, E14.5, E16.5, and E18.5). n = 6 mice per group. Scar bar: b 1 mm, d (upper) 200 µm, f, g, h 100 µm, d (lower), e 50 µm. Data were presented as the mean ± s.e.m. P values are from a one-way ANOVA test followed by Tukey’s post hoc test (c) or two-tailed unpaired Student’s t-test (d–h). Source data are provided as a Source Data file.
