Fig. 1: Homologous and cross-reactive antibodies induced by WA1 and variant infections. | Nature Communications

Fig. 1: Homologous and cross-reactive antibodies induced by WA1 and variant infections.

From: Primary exposure to SARS-CoV-2 variants elicits convergent epitope specificities, immunoglobulin V gene usage and public B cell clones

Fig. 1

A Antibody binding titers against multiple variants assessed by cell surface S-binding assay. The data are shown as geometric mean with geometric standard deviation. Geometric means of median fluorescence intensity (GeoMFI) are shown on top. GeoMFI for homologous titers were used as reference to calculate fold-change in GeoMFI for the other variants. Statistical significance was determined by Friedman’s test followed by Dunn’s test with correction for multiple comparisons. Separate serum aliquots were used to test binding of each variant, and the results represent a single measurement per sample. B Heatmap showing neutralizing antibody titers (reciprocal 50% inhibitory dilution, ID50) for each individual (N = 4 donors in WA1-infected, 7 donors in Beta-infected, and 2 donors in Gamma-infected Supplementary Table 1) labeled on the left against each variant indicated on the top. The results represent a single measurement per sample. C Epitope mapping on homologous Spike by competition assay using surface plasmon resonance. Antibody CB6 (RBD-B epitope) does not bind to Beta and Gamma Spike; LY-COV555 (RBD-E epitope) does not bind to Gamma Spike; A19-30.1 (RBD-I epitope) does not bind to Beta Spike; and CR3022 (RBD-K epitope) does not bind to Gamma Spike; therefore, competition was not measured at these sites. Measurements for each individual were performed in duplicate and averaged. WA1-infected samples mapped on WA1 Spike are shown in grey, Beta-infected samples mapped on Beta Spike in orange, and Gamma-infected donors mapped on Gamma Spike in blue. Graphs show the mean for each epitope and group with standard deviation. Statistical analysis was performed using an unpaired, two-tailed t-test. D Representative FACS plots for each condition of no stim or Spike A-stimulated CD4 + (left) and CD8 + (right) memory T cells. E CD4 (left) and CD8 (right) T cell responses to WA1 Spike peptide pools A + B, selected pools containing altered variant peptides and control pool containing correspondent peptides for each variant pool. Results represent a single measurement per sample. Graphs show the mean for each condition with standard deviation. For A, C and E, symbol shapes indicate each donor, as shown in legend on panel B. For panel E only, the Beta-infected donors are from a different cohort. Source data are provided as a Source Data file.

Back to article page