Fig. 1: IL-10 depletion aggravates neutrophil-predominant inflammation following LPS challenge.
a Experimental strategy for morphological and functional analysis of neutrophils after ALI. WT and Il-10−/− mice were treated nasally with 10 mg/kg LPS or PBS, followed by analyses at 6 h, 12 h, 24 h, 4 d and 7 d after administration. Some figure elements were created with BioRender.com. b–e Animals were euthanized and BALF was evaluated for total protein (b), total cell number (d), neutrophil, macrophage and lymphocyte numbers (e) at 6 h, 12 h, 24 h, n = 6 in each group; as well as H&E cell staining (d) at 24 h after LPS challenge. Scale bars, 20 μm. f–m Production of cytokines in BALF and serum was evaluated for IL-6 (f, n = 4, 5, 5, 5 in each time point from WT and Il-10−/− group both in BALF and serum), KC (g, n = 4, 5, 5, 5 in each time point from WT and Il-10−/− group both in BALF and serum), TNF (h, n = 4, 5, 5, 5 in each time point from WT and Il-10−/− group both in BALF and serum), G-CSF (i, n = 4 in each group both in BALF and serum), IL-12p70 (j, n = 5 in each group both in BALF and serum), IFN-γ (k, n = 4, 5, 5, 5 in each time point from WT and Il-10−/− group in BALF; n = 4, 5, 4, 4 in each time point from WT and Il-10−/− group in serum), IL-1β (l, n = 4, 5, 5, 5 in each time point from WT and Il-10−/− group in BALF; n = 4, 5, 4, 4 in each time point from WT and Il-10−/− group in serum) and IL-17A (m, n = 4 in each group both in BALF and serum). All samples were biologically independent and three or more independent experiments with similar results were performed. Data are presented as mean ± SEM and analyzed with a 95% confidence interval. Statistical analysis was performed using two-tailed unpaired Student t test. Source data are provided as a Source Data file.
