Fig. 2: IL-10 deficiency exacerbates the extensive lung injury induced by LPS. | Nature Communications

Fig. 2: IL-10 deficiency exacerbates the extensive lung injury induced by LPS.

From: Locally organised and activated Fth1hi neutrophils aggravate inflammation of acute lung injury in an IL-10-dependent manner

Fig. 2

a Representative images of lungs and H&E-stained tissue sections at 24 h after LPS stimulation. Scale bars in the upper panel, 1 mm; scale bars in the below panel, 50 µm. b Lung damage was determined by 5 pathophysiological features to obtain the total injury score, n = 6 in each group. c The permeability of alveolocapillary membrane was evaluated by the lung wet/dry (W/D) ratio, n = 6 in each group. d–i Immunohistochemical staining and quantitative analysis of Ly6G (d, e, n = 7 in each group), MPO (f–g, n = 7 in each group) and NE (h, i, n = 6 in each group) in mouse lung samples. ns, not significant. All samples were biologically independent and three or more independent experiments with similar results were performed. Data are presented as mean ± SEM and analyzed with a 95% confidence interval. Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s post hoc test. Source data are provided as a Source Data file.

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