Fig. 6: 4EBP1 activation increases satellite cell number and activity.
From: Muscle 4EBP1 activation modifies the structure and function of the neuromuscular junction in mice
a Representative confocal images of PAX7 (red), EdU (green), DAPI (blue) and Laminin (white) staining of cross-sections of the gastrocnemius muscle from 3-month-old male mice (3mo Control and 4EBP1mt-muscle, n = 4 per group). Solid Arrows = EdU and PAX7 double-positive satellite cells. Scale bar = 20 μm. b, c Quantification of the number of Pax7-positive nuclei normalized to (b) area and (c) number of myofibers. d, e Quantification of the percentages of (d) EdU and PAX7 double-positive cell and (e) EdU-positive nuclei. Sample size: PAX7 and EdU staining in cross section (Fig. 6b–e), 3mo Control and 4EBP1mt-muscle Male, n = 4 per group; on average, 400 myofibers sampled per animal. Statistical significance: PAX7 and EdU staining in cross section (Fig. 6b–e), Two-tailed unpaired student t-test. Only a P value of less than 0.05 would be labeled in the figure.
