Fig. 7: 4EBP1 activation increases the rate of AChR turnover.
From: Muscle 4EBP1 activation modifies the structure and function of the neuromuscular junction in mice
a Representative confocal images of BTX (green), EdU (red) and DAPI (blue) staining of longitudinal sections of the quadriceps muscle from 3-month-old male mice (3mo Control and 4EBP1mt-muscle, n = 4 per group). Open arrows = EdU-positive post-synaptic myonuclei; Solid arrows = EdU-positive non-synaptic myonuclei. Scale bar = 50 μm. b Representative confocal images showing ‘Old AChR’ labeled with BTX-AF647 (magenta) and ‘New AChR’ with BTX-AF488 (green) from longitudinal sections of the tibialis anterior muscle of 3-month-old Control (n = 3) and 4EBP1mt-muscle (n = 3) male mice as well as control mice treated with BaCl2 to induce muscle damage (3mo BaCl2, n = 3). Scale bar = 20 μm. c, d Quantification of the percentage of (c) EdU-positive post-synaptic nuclei and (d) NMJ with EdU-positive nuclei. Sample size: 3mo Control and 4EBP1mt-muscle Male, n = 4 per group; on average, 15,000 myonuclei were sampled per animal. Statistical significance was determined by two-tailed unpaired student t-test. e Quantification of AChR turnover in the NMJs. Sample size: 3mo Control, 4EBP1mt-muscle, Control treated with BaCl2 Male, n = 3 per group, on average 20 NMJs were sampled per animal. Statistical significance was determined by one-way ANOVA followed by Tukey post-hoc pairwise.
