Fig. 5: Men enzyme activity pairs SREBP to sleep.
From: SREBP modulates the NADP+/NADPH cycle to control night sleep in Drosophila
a NADP+/NADPH ratio at ZT12 by HILIC-MS/MS in control (n = 10), Cyfip85.1/+ (n = 10), Cyfip85.1/Srebp189 (n = 5), Srebp189/+ (n = 5), Cyfip85.1/MenBG02790 (n = 5), MenBG02790+ (n = 5). n = independent samples, pool of 45 fly heads. One-way ANOVA test and Sidak’s multiple comparisons test, control vs. Cyfip85.1/+ *p = 0.0359; Cyfip85.1/+ vs. Cyfip85.1/MenBG0279 *p = 0.0188; Cyfip85.1/MenBG02790 vs. MenBG02790 **p = 0.0075; control vs. MenBG02790+ ***p = 0.0001. Mean ± S.E.M. b Men activity at ZT10, in control (n = 5), Cyfip85.1/+ (n = 6) and Cyfip85.1/Srebp189 (n = 6). n = independent samples, pool of 10 fly heads. Two-way repeated measures ANOVA and Tukey’s multiple comparisons test, time effect ****p < 0.0001, genotype effect *p = 0.0137, interaction ****p < 0.0001, F(138, 966) = 11.14; control vs. Cyfip85.1/+ *p = 0.0304; Cyfip85.1/+ vs. Cyfip85.1/Srebp189 **p = 0.0026; control vs. Cyfip85.1/Srebp189 p = 0.57, n.s. = not significant. Mean ± S.E.M. c Quantification of night-time sleep in control (n = 71), Cyfip85.1/+ (n = 51), Cyfip85.1/MenBG02790 (n = 60) and MenBG02790/+ (n = 35) flies. One-way ANOVA and Sidak’s multiple comparisons test, control vs. MenBG02790/+ *p = 0.046; Cyfip85.1/+ vs. Cyfip85.1/MenBG0279 ***p = 0.0007; control vs. Cyfip85.1/MenBG0279 **p = 0.0031; control vs. Cyfip85.1/+ and Cyfip85.1/MenBG0279 vs. MenBG02790/+ ****p < 0.0001. Mean ± S.E.M. Source data are provided as a Source Data file. d Model.
