Fig. 6: Remission of jejunal mucositis by thiolated EcN.
From: Chemical reaction-mediated covalent localization of bacteria
a Schematic illustration of promoted remission of jejunal mucositis by covalent bonding-mediated localization of thiolated EcN. Mice were daily gavaged with PBS, 1 × 108 CFUs unmodified EcN, or equivalent EcN@SH for 5 days and a dose of 150 mg/kg per mouse of 5-FU was injected intraperitoneally on day 3. Mice were euthanatized at day 6 for sample collection. Healthy mice were used as a control. b Average intestinal length after treatment. c Level of TNF-α in serum measured by commercially available ELISA kits. d Typical images of H&E staining of the jejunum. Blue arrows show inflammation and red arrows indicate vacuolization and edema. Scale bar: 100 µm. Quantitative analysis of e villus length, f crypt depth, and g villus/crypt ratio in the jejunum. h Immunofluorescence images of ZO-1 and occludin staining of the jejunum. Scale bar: 100 µm. Data are presented as mean values ± SD (n = 5 mice). Significance was assessed using one-way ANOVA analysis followed by Fisher’s LSD multiple comparisons, giving P values, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns no significance. Source data and the exact P values are provided in the Source Data file.
