Fig. 2: Model of the complex of Singapore16 and Hong Kong14 N2 NAs with 1G01 Fab and negative-stain electron microscopy of antibodies 1G01, 1G04, and 1E01 bound to Singapore16 N2 NA. | Nature Communications

Fig. 2: Model of the complex of Singapore16 and Hong Kong14 N2 NAs with 1G01 Fab and negative-stain electron microscopy of antibodies 1G01, 1G04, and 1E01 bound to Singapore16 N2 NA.

From: Antibodies targeting the neuraminidase active site inhibit influenza H3N2 viruses with an S245N glycosylation site

Fig. 2

A The Singapore16 N2 NA was modeled with a mannose-9 glycan at Asn245. The 1G01 Fab is able to interact with the N2 NA (left). The glycan can be accommodated without steric clash with the 1G01 Fab. A model of the interaction of 1G01 Fab to Hong Kong14 N2 NA is also shown (right). NA protomers are colored green. Asn245 and attached glycans, as well as the ten residues that are not conserved between these two N2 NAs in their ectodomain (82–469) are shown in sticks and colored with yellow carbon, red oxygen and blue nitrogen atoms. The 1G01 Fab is colored brown (heavy chain) and gray (light chain). B Two-dimensional class averages of Singapore16 N2 NA in complex with 1G01, 1G04, and 1E01 Fabs (Top). Standard view (middle) and side view (bottom) of three-dimensional reconstruction of negative-stain EM maps of Singapore16 N2 NA tetramer (center, in green) in complex with four 1G01 Fabs (in orange), four 1G04 Fabs (in yellow), and 1E01 Fabs (in cyan), respectively (densities with a dimple on the periphery). The crystal structures of 1G01 Fab (PDB code 6Q23), 1G04 Fab (PDB code 6Q1Z), and 1E01 Fab (PDB code 6Q20) and the Singapore16 N2 NA tetramer model were docked into the reconstruction, respectively, and the Fabs were adjusted individually to best fit the EM density.

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