Fig. 5: MT1-MMP inhibition suppresses SARS-CoV-2 infection in human organoids.
From: Control of SARS-CoV-2 infection by MT1-MMP-mediated shedding of ACE2
a–e The inhibitory effect of MT1-MMP knockdown on SARS-CoV-2 infectivity in primary cells and organoids. Primary human bronchial epithelial 3D cultures with an air-liquid interface (ALI) (n = 3 biologically independent experiments), human cardiac organoids formed by iPSC-derived cardiomyocytes (n = 3) and human liver organoids (n = 3) were lentivirally transduced with shRNA targeting MT1-MMP or control shRNA, following by the infection of SARS-CoV-2. d Air–liquid interface culture was pretreated with increasing dosages of 3A2 and infected with SARS-CoV-2. (n = 3 biologically independent experiments) e Human bronchial epithelial cells transduced with shMT1-MMP or shRNA control were infected with SARS-CoV-2 omicron variant. (n = 3 biologically independent experiments) Data are means ± S.E.M. of three independent repeats. Statistical analyses were performed by one way ANOVA for (d) and multiple unpaired two-tailed t-test for (a–c), e. Source data are provided as a Source Data file.
