Fig. 4: The movable range of the accessory proteins of RQT complex.
From: Decoding of the ubiquitin code for clearance of colliding ribosomes by the RQT complex
a The time-lapse HS-AFM images of the Slh1/Cue3 complex. b Time course of the distance between Slh1 and Cue3 as described in the schematic diagram. The distance was calculated for each frame per 0.1 s. c The time-lapse HS-AFM images of the Slh1/Rqt4 complex. d Time course of the distance between center of Slh1 and the most distant point of Rqt4 from center of Slh1 as described in the schematic diagram. The distance was calculated for each frame per 0.1 s. e The histogram of the Slh1-Cue3 distance (b) and the Slh1-end of Rqt4 distance (d). f The 2D plot of the center positions of Cue3 (magenta) and Slh1 (cyan) of all frames on the Slh1/Cue3 HS-AFM image at 0 s frame. (g) The 2D plot of the most distant positions of Rqt4 (magenta) and the center position of Slh1 (cyan) of all frames on the Slh1/Rqt4 HS-AFM image at 0 s frame. All experiments were performed at least twice with highly reproducible results.
