Fig. 2: Transcriptomic changes upon perfusion.
From: Large-scale perfused tissues via synthetic 3D soft microfluidics
a Combined dataset UMAP (control, non-perfused and perfused samples). b Combined dataset UMAP with neuroepithelial cells (NE), pluripotent-neuroepithelial transitioning cells (P-NE), proliferating cells with medium mitochondrial (mito.) content, pluripotent cells (P) with low glycolysis (glyc.) and medium mitochondrial content, glycolytic pluripotent cells with low mitochondrial content, highly glycolytic pluripotent cells, a highly glycolytic hypoxic (hypo.) identity and a stressed cluster. c Pseudotime trajectory on combined dataset UMAP. d Cluster-specific expressions of selected marker genes. Color bar represents gene expression values, normalized and centered. Sample fractions for each identified cluster. e GO enrichment analysis for key processes upregulated in perfused and non-perfused samples.
