Fig. 3: PRSS2 represses Tsp-1 by activating Rac downstream of LRP1.

A Dot plot of GTP-bound Rac and Rho in WI38 cells that were untreated (control), treated with CM from 293T cells transfected with empty vector (pCMV) or PRSS2 (PRSS2) (n = 3; P value calculated via two-sided Student’s t test). B Western blot of Tsp-1 and actin expression in WI38 cells that were untreated (–) or treated with PRSS2 (+) in the absence (–) and presence (+) of a small molecule inhibitor of Rac1 NSC23766 (n = 3). C Dot plot of quantitation of Tsp-1 western blots from all replicates of WI38 that were untreated (–) or treated with PRSS2 (+) in the absence (–) and presence (+) of NSC23766 (n = 3; P value calculated via two-sided Student’s t test). D Western blot of phospho c-Jun, total c-Jun and actin in WI38 cells that were treated with saline (–), PRSS2, Rac1 inhibitor NSC23766, or PRSS2 and NSC23766 in combination (n = 3). E Dot plot of quantitation of phospho c-Jun, total c-Jun, and actin in WI38 cells that were treated with saline (–), PRSS2, Rac1 inhibitor NSC23766, or PRSS2 and NSC23766 in combination (P values calculated by two-sided ANOVA; n = 3). F Western blot of phosphor-JNK, total JNK and actin in WI38 cells that were treated with saline (–), PRSS2, Rac1 inhibitor NSC23766, or PRSS2 and NSC23766 in combination (n = 5). G Dot plot of quantitation of phospho-JNK, total JNK and actin in WI38 cells that were treated with saline (–), PRSS2, Rac1 inhibitor NSC23766, or PRSS2 and NSC23766 in combination (P values calculated by two-sided ANOVA; n = 5). H Western blot of Tsp-1 and actin in WI38 cells that were treated with saline (–), PRSS2, JNK1 inhibitor III, or PRSS2 and JNK1 inhibitor III in combination (n = 3). I Schematic diagram of PRSS2 repression of Tsp-1 via LRP1-Rac signaling.