Fig. 1: Loss of AIF1 prevents obesity and insulin resistance induced by HFD. | Nature Communications

Fig. 1: Loss of AIF1 prevents obesity and insulin resistance induced by HFD.

From: Increased adipose catecholamine levels and protection from obesity with loss of Allograft Inflammatory Factor-1

Fig. 1

WT and Aif1−/− (KO) mice were assigned to chow (CD) or high-fat diet (HFD) starting at 8 weeks of age. a, b Computerized tomographic scans were performed after 16 weeks on diet. a Whole body projections show skeletal and soft tissue signals. b False-colored cross-sectional samples indicate visceral (pink) and subcutaneous (yellow) adipose tissues, plus lean (light blue) and skeletal (green) elements. c Body weight measured at the indicated time points after starting on CD or HFD (n: WT-CD, 9; KO-CD, 6; WT-HFD, 9; KO-HFD, 9). d Fat and lean masses measured by magnetic resonance imaging after 16 weeks on diet (n: WT-CD, 8; KO-CD, 7; WT-HFD, 8; KO-HFD, 5). e–g AIF1 expression after 16 weeks on diet assessed by Western blot in eWAT (e), iWAT (f), and BAT (g). The experiment was repeated 3 times with similar results. h–k Insulin resistance in WT and Aif1−/− mice after 16 weeks of CD or HFD. h Glucose tolerance test (n: WT-CD, 5; KO-CD, 5; WT-HFD, 4; KO-HFD, 5). Arrows indicate the effect of loss of AIF1 for a given diet. i–k Euglycemic, hyperinsulinemic clamp studies, showing glucose clearance rate (i), glucose infusion rate (j), and hepatic glucose production (HGP) (n: WT-HFD, 8; KO-HFD, 8) (k). Data are presented as mean ± s.e.m. Differences were evaluated by two-way ANOVA followed by Sidak’s multiple comparison test (c), Tukey’s multiple comparison test (d, h) and for i–k, two-sided Mann–Whitney U-test. n = the number of biologically independent animals. Source data are provided as a Source Data file.

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