Fig. 2: Mice lacking AIF1 expend more energy in both ambient and thermoneutral conditions, independent of changes in activity or temperature. | Nature Communications

Fig. 2: Mice lacking AIF1 expend more energy in both ambient and thermoneutral conditions, independent of changes in activity or temperature.

From: Increased adipose catecholamine levels and protection from obesity with loss of Allograft Inflammatory Factor-1

Fig. 2

Metabolic characteristics of AIF1-deficient and control mice fed with CD or HFD for 16 weeks were measured using metabolic cages at ambient temperature (22 °C), at thermoneutrality (30 °C), or at reduced temperature (10 °C). a Energy expenditure (EE) at 22 °C, showing mean values collected over 4 days (left panel) and over a 24 h light-dark cycle (right panel) (n: WT-CD, 8; KO-CD, 6; WT-HFD, 6; KO-HFD, 6). b, c Similar assessments, but with mice housed at 30 °C (b) (n: WT-CD, 6; KO-CD, 5; WT-HFD, 4; KO-HFD, 4) or 10 °C (c) (n: WT-CD, 4; KO-CD, 3; WT-HFD, 4; KO-HFD, 3). d Physical activity measured by beam breaks, or e temperature, showing mean values collected over 4 days at 22 °C (n: WT-CD, 8; KO-CD, 6; WT-HFD, 6; KO-HFD, 6). f Physical activity measured by beam breaks, or g, temperature, showing mean values collected over 4 days at 30 °C (n: WT-CD, 6; KO-CD, 5; WT-HFD, 4; KO-HFD, 4). h Physical activity measured by beam breaks, or i temperature, showing mean values collected over 5 days at 10 °C (n: WT-CD, 4; KO-CD, 3; WT-HFD, 4; KO-HFD, 3). Data are presented as mean ± s.e.m.; significance is assessed by unpaired two-sided Student’s t-test. Data are representative of three similar experiments. n = number of biologically independent animals. Source data are provided as a Source Data file.

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