Fig. 4: AIF1 expression in bone marrow-derived cells affects obesity resistance.

a Total bone marrow cells from WT or Aif1^−/− donor mice (age 6–8 weeks) were transferred to irradiated WT or Aif1^−/− recipient mice (age 4–6 weeks). After 6 weeks of recovery, mice were randomly assigned to CD or HFD for 6 weeks and weighed regularly. Key indicates donor-recipient and diet, e.g., WT-KO CD. a (left) %weight gain; (right), fat and lean masses, assessed by MRI after 6 weeks on diet. n: WT-KO CD, 5; KO-WT CD, 4; WT-KO HFD, 4; KO-WT HFD, 5. Data were evaluated by two-way ANOVA followed by Tukey’s multiple comparison test and presented as mean ± s.e.m. b–d RNA-seq data obtained from immortalized BMMs generated from male WT and Aif1^−/− mice (n = 3 biologically independent animals). b Unsupervised hierarchical clustering analysis. c Bioamine degradation pathways predicted by GO analysis. d heat map representing the expression of key genes in the NE degradation pathway. e (upper) expression of Aif1 and NE catabolism genes (Maoa, Aldh1/1, and Aldh1/2) in BMMs validated by qRT-PCR (top) (n: WT, 9; KO, 8, pooled from 3 independent isolations and presented as mean ± s.e.m.); e (lower), protein expression of AIF1 and MAOA in BMMs assessed by immunoblotting, with β-actin shown as a loading control (n: WT, 5; KO, 4, pooled from 3 independent cell isolations and representative of 3 similar experiments). f MAOA activity measured in WT vs. Aif1^−/− BMMs with or without MAOA inhibitor (MAOA-I (clorgylline, 10 μM)). RFU relative fluorescent units (n = 2, graph shows mean ± s.e.m. and is representative of two similar experiments). g Catecholamine clearance–NE (2 μM) was added to WT and Aif1^−/− BMMs in culture and cellular NE levels were assessed after 2 h. MAOA-I, clorgylline, 10 μM. n = 3 per group for baseline measurement and n = 4 per group for other conditions and are representative of 2 similar experiments. Data are presented as mean ± s.e.m. Significance was assessed by one-way ANOVA with Tukey’s multiple comparisons test. Source data are provided as a Source Data file.