Fig. 5: Loss of AIF1 impairs catecholamine catabolic enzyme expression and activity in ATMs.

a–c Expression of key genes in NE degradation, assessed in WT vs. Aif1^−/− in ATMs by qRT-PCR.ATMs were sorted from pooled SVF of BAT (a), iWAT (b), and eWAT (c) obtained from WT or Aif1^−/− mice (n = 3 (BAT, eWAT) or 2 (iWAT) independent pools, each pool from 5 mice) after 16 weeks of HFD feeding; heat map (left), bar graph (right). Data pooled from 3 independent experiments d–g Flow cytometric analysis of MAOA expression in CD45 + CD11b + F4/80+ cells from SVF BAT (n: WT, 3; KO, 3) (d), iWAT (n: WT, 5; KO, 4) (e) and eWAT(n: WT, 5; KO, 4) (f), plus a summary of quantification (g). Data are representative of 2 similar experiments. h NE levels measured in ex vivo ATM culture medium (left panel) and cell lysates (right panel); SVF from 5 mice in each group were pooled and sorted to obtain CD45 + CD11b + F4/80+ cells, and NE was measured in 2 or 3 technical replicates). Data are presented as mean ± s.e.m. Statistical significance was assessed by unpaired two-sided t-test (a–c and g) and two-way ANOVA using Tukey’s multiple comparisons test (h). i Linear regression analysis of AIF1 and MAOA or ALDH1L2 expression in subcutaneous adipose tissues of clinical participants with excess weight or obesity (n = 11), with significance assessed by Pearson’s correlation. Curved lines indicate 95% confidence intervals. n represents a number of biologically independent animal or human participants unless otherwise noted. Source data are provided as a Source Data file.