Fig. 5: The projection of NAcC D1-MSNs to LH and the infulence of morphine withdrawal memory on this projection. | Nature Communications

Fig. 5: The projection of NAcC D1-MSNs to LH and the infulence of morphine withdrawal memory on this projection.

From: Nucleus accumbens circuit disinhibits lateral hypothalamus glutamatergic neurons contributing to morphine withdrawal memory in male mice

Fig. 5: The projection of NAcC D1-MSNs to LH and the infulence of morphine withdrawal memory on this projection.

a Top: the diagram of virus injection into the NAcC (AAV-DIO-ChR2-mCherry) and LH (AAV-mDIx-EGFP and AAV-fDIO-mCherry) in D1-Cre::Vglut2-Flpo mice. Middle: the expression of ChR2-mCherry in NAcC, the expression of mDlx-EGFP and fDIO-mCherry in LH (Scale bar, 200 μm). Bottom: the schematics of the whole-cell patch recording. b Left: the representative IPSCs evoked in connected and non-connected LH glutamatergic neurons by the light stimulation (blue light, 470 nm, 2 ms). Right: the percentage of connected and non-connected LH glutamatergic neurons (n = 25 cells of 5 mice). c Left: the representative IPSCs evoked in connected and non-connected LH GABAergic neurons by light stimulation (blue light, 470 nm, 2 ms). PTX (100 μM) blocked light-evoked IPSCs. Right: the percentage of connected and non-connected LH GABAergic neurons (n = 39 cells of 5 mice). d The experimental timeline. e Top: the diagram of virus injection into the NAcC (AAV-DIO-ChR2-mCherry) and LH (AAV-mDIx-EGFP) in D1-Cre mice; Middle: the expression of ChR2-mCherry in NAcC and LH, the expression of mDlx-EGFP in the LH (Scale bar, 200 μm). Bottom: the schematics of the whole-cell patch recording approach. f The average CPA score in SS (n = 8) and MN (n = 8) groups. Two-way ANOVA, drug treatment factor, F (1,14) = 69.12, p < 0.0001; test condition factor, F (1,14) = 40.47, p < 0.0001; drug treatment × test condition, F (1,14) = 45.20, p < 0.0001. g Left: the representative paired-pulse ratio (PPR) of light-evoked (470 nm, 2 ms) IPSCs evoked in LH GABAergic neurons in SS and MN groups. Right: average PPR in SS (n = 16 cells) and MN (n = 20 cells) groups. Unpaired t test, p < 0.0001. Mean ± SEMs. ****p < 0.0001.

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