Fig. 1: TNBC and ILC differ in histology and quantity of CAFs. | Nature Communications

Fig. 1: TNBC and ILC differ in histology and quantity of CAFs.

From: CD26-negative and CD26-positive tissue-resident fibroblasts contribute to functionally distinct CAF subpopulations in breast cancer

Fig. 1

A HE and immunohistochemical stainings of non-tumor bearing control mammary glands and tumors derived from ILC mouse models (WEPtn and WEH1047R) and TNBC mouse models (WB1P and WB1P-Myc) for fibroblast markers (platelet-derived growth factor receptor beta: PDGFRβ, alpha smooth muscle actin: SMA, vimentin), collagen (Masson trichrome) and epithelial cells (epithelial cell adhesion molecule: EpCAM and E-cadherin). Images are representatives of n = 4 mice per tumor model. Scale bars are 50 um. B Flow cytometry analysis of tumor composition at indicated stages of tumor development (WEPtn and WEH1047R) or tumor dimensions in mm (WB1P and WB1P-Myc mice), minimum of n = 6 mice per time point. Mean percentage for every population is shown with error bars reflecting the standard error of the mean (SEM). Mammary epithelial cells (tumor cells) were defined as EpCAM+/CD49f+/CD31−/CD45−/PDGFRβ−. Endothelial cells were defined as CD31+/EpCAM−/CD45−. Immune cells were defined as CD45+/CD31−/EpCAM−. Fibroblasts in the WEPtn and WEH1047R models were defined as EpCAM−/CD45−/CD31−/PDGFRβ+. Fibroblasts in the WB1P and WB1P-Myc models were defined as EpCAM−/CD49f−/CD45−/CD31−/PDGFRβ+. Source data are provided in source data file.

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