Fig. 3: EXO1 limits DNA replication stress and DNA damage induced by formaldehyde.
From: A CRISPR-Cas9 screen identifies EXO1 as a formaldehyde resistance gene
a Protein levels of EXO1, pChk1 (pSer 345), and Chk1 in RPE-1 WT, KO 7, KO 11, and AAVS1 complemented cells with or without 100 µM formaldehyde treatment for 18 h, with housekeeping genes (β-actin or vinculin) as loading controls. b CldU track length of DNA fibers from AAVS1 complemented cells with or without 100 µM formaldehyde treatment for 18 h. Data are shown with mean ± SEM from three independent experiments. ns: non-significant, ****p < 0.0001 (one-way ANOVA, followed by Kruskal–Wallis test). EV: AAVS1 + Empty Vector. c CldU track length of DNA fibers in RPE-1 WT and EXO1 KO 11 cells with the indicated treatment. Data are shown with mean ± SEM from three independent experiments. ns: non-significant, *p < 0.1. (One-way ANOVA, followed by Kruskal–Wallis test).
