Fig. 1: Enrichment of telomere-containing genomic DNA fragments using telobaits for PacBio HiFi sequencing. | Nature Communications

Fig. 1: Enrichment of telomere-containing genomic DNA fragments using telobaits for PacBio HiFi sequencing.

From: High-throughput telomere length measurement at nucleotide resolution using the PacBio high fidelity sequencing platform

Fig. 1

a Schematic illustration of the key steps in the enrichment of telomere repeat-containing genomic DNA fragments for PacBio HiFi sequencing. b Teloblot results showing that the telomere-containing genomic DNA fragments enriched following telobaits ligation (without trimming using RsaI and HinfI restriction endonucleases) and eluted after EcoRI digestion have similar length distributions as genomic DNA fragments digested directly using EcoRI restriction endonuclease. Representative teloblot results from one of the two independent experiments are shown (Source Data 1).

Back to article page