Fig. 2: Telomere length measurement in culture cells using PacBio HiFi sequencing.

a Schematic illustration for the estimation of raw telomere length or telomere length with or without the inclusion of heterogenous TVSs in sequencing reads, respectively. b, c Histograms and violin plots of telomere length distribution in HCT116, 293T, T24, IMR90 (PD47), and WI38 (PD40) cells obtained from two biological replicates (Rep.) in a single sequencing run. PD population doubling. Within each violin, the white center circle denotes the median value, the bounds of box represent the 25th to 75th percentile values, the whiskers represent adjacent values within 1.5 interquartile range, the ends of the whiskers depict the minimum and maximum values within the range, and the violin shape reflect the kernel density plot of the entire dataset. Source data are provided in Source Data 2. d. Table showing the characteristics of telomere length distribution in HCT116, 293T, T24, IMR90 (PD47) and WI38 (PD40) cells obtained from two biological replicates as shown in the violin plot in c. e Beeswarm plot showing the over-representation of extremely short telomeres (<1000 bp) in sequencing reads, specifically from T24 cells. Source data are provided in Source Data 2. f Bar graph showing the over-representation of extremely short telomeres (<1000 bp) in sequencing reads, specifically in T24 cells after normalization. g Teloblot of HCT116, 293T, T24, IMR90 (PD47), and WI38 (PD40) genomic DNA digested with the same HinfI and RsaI restriction endonucleases that were used to trim down the genomic DNA fragment during telomere enrichment for PacBio HiFi sequencing. Representative results from one of the two independent experiments are shown (Source Data 1).